Interactive Tool

Reconstitution Calculator

Calculate the exact volume of bacteriostatic water needed for your target concentration, plus the precise draw volume for each dose.
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8-Step Guide
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Auto-Calculated
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5 Concentrations
Reconstitution Protocol

8-Step Guide

Follow these steps in order for safe, accurate reconstitution. Click each step to expand.

Thoroughly wash hands with soap and water for at least 20 seconds. Dry with a clean paper towel. This is your first and most important contamination barrier.

Alternatively, use nitrile gloves throughout the entire process.

Lay out: peptide vial (lyophilized powder), bacteriostatic water (BAC water), insulin syringe (1 mL / U-100), alcohol swabs (70% isopropyl), and a clean flat surface.

Organize left-to-right in the order you'll use them. Never rush โ€” precision matters.

Use a fresh alcohol swab to clean the rubber stopper on both your peptide vial and your BAC water vial. Wipe in one direction and allow 10โ€“15 seconds to air dry completely.

Never blow on the stopper to dry it โ€” this introduces bacteria from your breath.
Always let the alcohol fully dry before inserting the needle.

Insert the syringe needle into your BAC water vial at a 45ยฐ angle. Invert the vial and slowly pull back the plunger to draw the calculated volume of BAC water (from the calculator below).

If you see air bubbles, gently tap the syringe and push the plunger slightly to expel them.

Insert the needle into the peptide vial. Aim the stream of BAC water at the inside glass wall โ€” do NOT squirt directly onto the powder. Allow water to trickle down the side.

The side-wall technique prevents degrading the peptide's delicate molecular structure.
Never inject BAC water forcefully straight onto the lyophilized powder.

Place the vial between your palms and gently roll back and forth. Do NOT shake or vortex the vial. The solution should dissolve clear โ€” if it remains cloudy, give it a few more minutes.

Most peptides dissolve within 30โ€“60 seconds of gentle rolling. Some may take up to 5 minutes.
Shaking can cause foaming which degrades peptide activity.

Hold the vial up to light and inspect for clarity. The solution should be completely clear and colourless (some peptides may have a very faint yellow tint โ€” this is normal).

Discard the vial immediately if you see visible particles, cloudiness, or unusual colouration.
A cloudy solution may indicate contamination or improper mixing.

Label the vial with the peptide name, date reconstituted, concentration (mcg/mL), and your initials. Store in the refrigerator (2โ€“8ยฐC). Most BAC water reconstitutions remain stable for 4โ€“6 weeks.

Avoid the refrigerator door (temperature fluctuates). Store in the main body of the fridge.

DRAW & DOSE CALCULATOR

Dose Of Peptide

Peptide Strength

Bacteriostatic Water

0.1 mg

PEPTIDE DOSE

5 units

DRAW SYRINGE TO

10 doses

VIAL CONTAINS

2 mg/mL

CONCENTRATION

Educational Disclaimer: This calculator is provided for educational and research purposes only. Results are mathematical approximations โ€” always verify calculations independently. House of Peptides does not provide medical advice, diagnosis, or treatment guidance. Consult a qualified healthcare professional before using any peptide compound.

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